Some prepared by the writer over twelve months ago,
according to the suggestion of the latter physician, has since shown
no signs of decomposition, and is now as good as it was then. For
qualitative purposes the solution may be prepared thus: Dissolve 35
gm. of recrystallized cupric sulphate and 200 c.c. of pure glycerin in
100 c.c. of distilled water. Dissolve separately 80 gm. of caustic
soda in 400 c.c. of water. Mix the solutions and boil for a quarter of
an hour. A small amount of reduction from impurity in the glycerin
takes place. Allow to stand till clear, decant, and dilute to 1,250
c.c. Ten cubic centimeters will then equal roughly 5 centigrammes of
glucose. For exact quantitative determination it is necessary to
standardize the solution with pure anhydrous dextrose.
To a practiced operator the indications yielded by the use of this
test are of great value; but beginners are exceedingly liable to
mistake its various reactions, and to report the urine as saccharine
when normal traces only of sugar are present.
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