Cool the evolved gas by placing the bottle in
cold water, adjust the levels of the water in the tube and reservoir
(to obviate a correction for pressure), and read off the percentage of
urea in terms of which the tube is graduated. Stale urine, the urea of
which has largely been converted into ammonic carbonate, still yields
a very fair result, that salt being also completely split up by the
powerful oxidant employed. Should the urine contain albumen, it is
advisable to remove it by boiling and filtering, as, although only
slowly decomposed by the hypobromite solution, it communicates to the
liquid such a tendency to froth that the disengagement of the nitrogen
is seriously impeded. Most of those alkaloids which might possibly be
present do not yield the gas when treated in this manner, and
therefore may be disregarded.
SUGAR.
Glucose, so characteristic of _diabetes mellitus_, is not difficult of
detection or estimation. The facility with which it reduces alkaline
cupric, argentic, bismuthous, ferric, mercuric salts, indigo and
potassic picrate and chromate solutions has been utilized for the
preparation of several ready methods for its determination.
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